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Free Content Study on Permeability of DMSO in Embryos of Red Seabream (Pagrus Major) by Capillary Electrophoresis

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The objectives were to investigate the permeability of DMSO to red seabream (Pagrus major) embryos by capillary electrophoresis and the effects of DMSO concentrations (5%40%, v/v) and immersion times (10, 30 and 60 min) on hatching rate and morphology. The results suggested the internal DMSO concentrations were positively related with the external concentrations and exposure times, while the hatching rate was negatively related. The hatching rate decreased drastically (<50%) after exposure in 35%, 20% and 15% DMSO for over 10, 30 and 60 min, respectively. In all groups, when hatching rate was >50%, the internal DMSO concentration was < 2%, which was still insufficient for successful cryopreservation. Morphological changes indicated the chorion was permeable to the cryoprotectant. A sign of dehydration in yolk were observed, for a significant decrease in the maximal yolk sac diameter. However, further research was needed to investigate whether the DMSO permeated into the yolk.

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Keywords: CAPILLARY ELECTROPHORESIS; DMSO PERMEABILITY; HATCHING RATE; MORPHOLOGY CHANGES; PAGRUS MAJOR

Document Type: Research Article

Publication date: 01 July 2011

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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