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Free Content Cryopreservation of Coconut (Cocos Nucifera L.) Zygotic Embryos by Vitrification

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The present study investigates the effect of preculture conditions, vitrification and unloading solutions on survival and regeneration of coconut zygotic embryos after cryopreservation. Among the seven plant vitrification solutions tested, PVS3 was found to be the most effective for regeneration of cryopreserved embryos. The optimal protocol involved preculture of embryos for 3 days on medium with 0.6 M sucrose, PVS3 treatment for 16 h, rapid cooling and rewarming and unloading in 1.2 M sucrose liquid medium for 1.5 h. Under these conditions, 70-80% survival (corresponding to size enlargement and weight gain) was observed with cryopreserved embryos and 20-25% of the plants regenerated (showing normal shoot and root growth) from cryopreserved embryos were established in pots.

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Keywords: COCONUT; COCOS NUCIFERA L; PRECULTURE; PVS3; UNLOADING; VITRIFICATION; ZYGOTIC EMBRYO

Document Type: Research Article

Publication date: 01 July 2011

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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