Vitrification and encapsulation-dehydration were tested for cryopreservation of Thymus moroderi Pau ex Martínez (Labiatae), an endemic plant from south-eastern Spain. For vitrification, shoot tips were loaded in a solution containing 0.4 M sucrose + 2 M glycerol for 20 min at room temperature, dehydrated in PVS2 solution for 0-105 min at 0°C, then immersed in liquid nitrogen (LN) for at least 1 day and rapidly rewarmed. The highest survival (71.4%) was obtained after 60 min PVS2 dehydration. Encapsulation-dehydration gave slightly lower results, with up to 50% explants survival. In the optimal protocol, donor plants were cold-hardened at 10°C for 5 weeks, excised shoot tips precultured for 48 h on MS medium with 0.08 M sucrose, encapsulated, pretreated in medium with 0.75 M sucrose for 19 h, desiccated to 22% moisture content (fresh weight basis), and immersed in LN. Vitrification thus appears more suitable than encapsulation-dehydration for cryopreservation of T. moroderi shoot tips.
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Document Type: Research Article
July 1, 2010
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.