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Free Content A Universal Self-Adaptive Time-Varying Function for Extracellular Concentration During Osmotic Shift for Curve-Fitting Permeability Coefficients of Cell Membrane

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A universal self-adaptive time-varying function of extracellular concentration history during osmotic shift for measuring cell membrane permeability was presented in this study. The feasibility and accuracy of the assumed function were verified based on the experimental data obtained from the microperfusion chamber method. It was found that the assumed function could always give out the very satisfactory coefficient of determination, and there were no significant differences between the hydraulic conductivity values fitted using the laser interferometer measured extracellular concentration profile and the predicted one by the assumed piecewise function (student's t test, p>0.05). Due to the adaptive feature of the assumed function for the concentration of extracellular solution, the function was suggest to be used for all the similar studies for measurement of cell membrane permeability by osmotic shift.

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Keywords: CURVE FITTING; EXTRACELLULAR CONCENTRATION; HYDRAULIC CONDUCTIVITY; OSMOTIC SHIFT

Document Type: Research Article

Publication date: May 1, 2010

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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