We evaluated the sensitivity of orthodox seeds of black alder (Alnus glutinosa (L.) Gaertn.) to extreme desiccation and/or to the ultra-low temperature of liquid nitrogen (LN; -196°C). The critical water content (WC) of desiccated seeds and the high-moisture freezing limit of seeds desiccated or imbibed to various WCs and frozen for 24 h or up to two years in LN was determined. Germination tests revealed that desiccation to 0.03 g H2O.g–1 dry mass was not detrimental to these seeds. Seeds tolerated LN freezing when the WC was 0.03-0.18 g H2O g–1 dm (nuts). Seeds desiccated to this WC and stored in LN for two years showed similar germination as seeds stored at -3°C for two years. Therefore, long-term cryopreservation of A. glutinosa seeds in genebanks is feasible.
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Document Type: Research Article
March 1, 2010
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.