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Home / Cryoletters, Volume 31, Number 1

Free Content Cryopreservation of Thymus moroderi by Droplet Vitrification

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Authors: Marco-Medina, Ana; Casas, José Luis; Swennen, Rony; Panis, Bart

Source: Cryoletters, Volume 31, Number 1, January 2010, pp. 14-23(10)

Publisher: Cryoletters

Thymus moroderi Pau ex Martínez (Labiatae) was successfully cryopreserved using the droplet vitrification method. After 20 min in loading solution at room temperature, shoot tips were dehydrated with PVS2 at 0°C for 30 min and immersed into LN. For thawing, shoot-tips were transferred into recovery solution for 15 min. A test of different recovery media revealed that the best results were obtained when the medium was supplement with 0.275 M BA.

Keywords: CRYOPRESERVATION; DROPLET VITRIFICATION; HYPERHYDRATION; IN VITRO CONSERVATION; THYMUS MORODERI

Document Type: Research Article

Publication date: 01 January 2010

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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