Skip to main content
padlock icon - secure page this page is secure

Free Content Cryopreservation Strategies for Cyathea australis (R. Br.) Domin.

Download Article:
 Download
(PDF 1,104.5 kb)
 
The influence of liquid nitrogen (LN) on the germination of C. australis spores and survival of gametophytes at various stages of development was investigated. Exposure to LN did not change the viability of mature spores (80%) but stimulated the germination of immature spores from 1.9% to 41%. Disinfection before cryopreservation contributed to loss of spore survival. However, some germination capacity was regained (48%) if the sterilized spores were enclosed in alginate capsules and subsequently exposed to osmotic desiccation and 5-hour air drying. Development of gametophytes derived from frozen and non-frozen spores was similar. Preculture factors (the period, type and abscisic acid treatment) affected gametophyte viability and growth. A two week preculture on agar significantly increased survival compared to preculture in a liquid medium. Addition of abscisic acid (ABA) to solid or liquid media stimulated explant survival. Highest viability (85%) of frozen-thawed gametophytes was achieved by a 2-week preculture in agar with 0.25 M sucrose and 10 M ABA. Gametophytes developed directly from spores grew and multiplied in vitro at a uniform rate. Young, intensively growing gametophytes and large, proliferating ones survived better (73-80%) following cryoexposure than mature, non proliferating gametophytes (50%). Less than one quarter of the explant surface was alive in 60-80% of the gametophytes that survived cryoexposure.

25 References.

No Supplementary Data.
No Article Media
No Metrics

Keywords: ABA; ENCAPSULATION; FLOW CYTOMETRY; GAMETOPHYTE; ROUGH TREE FERN; SPORE

Document Type: Research Article

Publication date: 01 November 2009

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more