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Free Content Cryo-Banking of Prunus mume Pollen and Its Application in Cross-Breeding

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The Mei (Prunus mume Sieb. et Zucc.) is one of the most widely used landscape plants and important germplasm resources in China. The study of pollen cryopreservation and the construction of a pollen cryo-bank have great importance in Mei research. From 2003 to 2007 we cryopreserved pollen from 51 Mei cultivars. In vitro germination of cryopreserved pollen was not significantly different from that of fresh pollen, even after four years of storage in liquid nitrogen. Cryopreserved pollen of 19 cultivars was used successfully for intraspecific hybridizations at Wuhan and Beijing in 2005 and 2006.

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Keywords: CROSS-BREEDING; CRYOPRESERVATION; POLLEN GERMINATION; PRUNUS MUME SIEB.ET ZUCC

Document Type: Research Article

Publication date: May 1, 2009

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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