Skip to main content
padlock icon - secure page this page is secure

Free Content Deployment of the encapsulation/dehydration protocol to cryopreserve diverse microalgae held at the Institute of Soil Biology, Academy of Sciences of the Czech Republic

Download Article:
 Download
(PDF 255.2 kb)
 
Twenty-seven strains of soil algae isolated from highly diverse provenances and habitats were assessed for their capacity to withstand cryopreservation using encapsulation/dehydration. Survival was assessed following the release of algae from alginate beads treated with sodium hexametaphosphate and regrowth was assessed using NAJA® Image Analysis. Regrowth occurred in 19 strains, with >50% survival being observed in 15. Algal tolerance to osmotic dehydration and evaporative desiccation was critical to the success of the method. Recovery in five out of the remaining eight recalcitrant strains was enhanced by substituting sorbitol for the osmotic pretreatment or by combining encapsulation with two-step controlled rate cooling.

7 References.

No Supplementary Data.
No Article Media
No Metrics

Keywords: CRYOPRESERVATION; CULTURE COLLECTIONS; CYANOBACTERIA; MICROALGAE; VITRIFICATION

Document Type: Regular Paper

Publication date: January 1, 2008

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more