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Free Content Determination of genetic stability in surviving apple shoots following cryopreservation by vitrification

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In vitro-grown apple shoot tips were successfully cryopreserved by vitrification, with an average survival and shoot formation of 80% and 76% respectively. Surviving shoots showed the same rate and regrowth patterns as those of non-treated controls. No significant differences (P<0.05) were observed in morphological characteristics, including shoot length, leaf shape, leaf width/length ratio and root length, between the control and cryopreserved shoots. No different microsatellite alleles and ISSR fragments were detected between control and cryopreserved shoots using twelve pairs of microsatellites and eleven ISSR primers. These results show that cryopreservation using vitrification is a practical method for the long-term storage of apple germplasm.

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Keywords: APPLE; CRYOPRESERVATION; GENETIC STABILITY; ISSR; MICROSATELLITE; VITRIFICATION

Document Type: Regular Paper

Publication date: 01 January 2008

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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