A simplified technique for the cryoprotection of dormant shoot-tips was developed for the germplasm conservation of herbaceous peony (Paeonia lactiflora Pall.). The highest post-thaw regrowth level was obtained from shoot-tips desiccated by air drying for 5 h. The post-thaw regrowth of P. lactiflora. var. 'Mikang' was the highest (74.9%) among the five genotypes tested. The regeneration level after cryopreservation was highest (66∼74%; average of 70%) for dormant shoot-tips collected from November to February. Shoot tips which were dissected from non-dormant buds in late March, however, showed very low post-thaw regrowth. Post-thaw regrowth of shoot-tips was promoted in MS medium containing 1 mg l−1 of gibberellic acid (GA3) and 0.5 mg l−1 of N6-benzyladenine (BA). The elongated shoots were rooted on a 1/4 MS medium containing 0.1 mg l−1 of -naphthalene acetic acid (NAA). In addition, random amplified polymorphic DNAs (RAPDs) assays suggested that the cryostorage treatment used here preserved the genetic fidelity of the peony dormant shoot-tips. This cryopreservation method appears to be promising for the conservation of herbaceous peony germplasm.
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Document Type: Research Article
Publication date: 01 May 2007
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.