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Free Content Freeze-drying of human platelets: influence of saccharide, freezing rate and cell concentration

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Freeze-drying of human platelets is one potentially ideal approach for long-term preservation of platelets. In this study, effects of concentration and type of saccharides, freezing rate and initial cell concentration on the recovery of freeze-dried platelets were investigated. Annexin V binding platelet activation assays, scanning electron microscopy and platelet aggregation upon thrombin (1 U/ml) addition were used to evaluate the effectiveness of platelet freeze-drying. The numerical recovery of freeze-dried platelets was reached as high as (93.0±5.2) % and the recovery of nonactived platelets was reached up to (85.7±3.4) % in the presence of 1%BSA and 20% trehalose. Frozen by shelf pre-cooling was the best way to freeze the sample in this study and the numerical recovery of freeze-dried platelets was reached (93.0±5.2) % at about 10 °C/min. When the platelet concentration was increased from 0.2 to 4×109 platelets/ml, recovery remained higher than 81.4%. The morphology of freeze-dried and rehydrated platelets was intact but a little rounder compared with fresh platelets. The maximum aggregation rate to thrombin (1 U/ml) of freeze-dried platelets was 83.9% of the fresh ones, but aggregation speed was 43.0% of the fresh ones. Further research on rehydration process and scale up are required.

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Keywords: CELL CONCENTRATION; COOLING RATE; FREEZE-DRYING; LYOPROTECTANT; PLATELETS

Document Type: Research Article

Publication date: May 1, 2007

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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