Skip to main content
padlock icon - secure page this page is secure

Free Content The effect of cold-acclimation on the water relations and freezing tolerance of Hordeum vulgare L.

Download Article:
 Download
(PDF 205.2 kb)
 
During a 5°C and a 5/-1°C cold acclimation (CA) regime there was a significant decline in the water potential of winter barley, and a concurrent decline in tissue water content of the 5/-1°C CA plants. Results of carbohydrate analysis illustrated a significant (P<0.001) accumulation of sucrose, fructose and glucose in the 5/−1°C CA plants, which was inversely correlated to water potential. Using an infrared imaging radiometer during a convection frost test the water release time (WRT) of 5/−1°C CA was demonstrated to be significantly (P<0.001) longer than that observed in non-cold acclimated plants. This observation is consistent with visual analysis of exotherm curves where the rate of cellular water release to extracellular ice is reduced in the 5/−1°C CA plants, compared to the non-cold acclimated plants. These biochemical and physiological changes were correlated to increased plant health following a non-lethal freezing test to −5°C, where non-cold acclimated plants produced 2.3 ± 0.33 tillers and 5°C and 5/−1°C A plants produced 2.4 ± 0.33 and 4.7 ± 0.67 tillers, respectively. Results from this study imply that cold acclimation leads to changes in the physical state of water that result in a less osmotically responsive cellular environment and subsequently significantly less damage to meristematic tissue.

18 References.

No Supplementary Data.
No Article Media
No Metrics

Keywords: CARBOHYDRATE ANALYSIS; INFRARED IMAGING RADIOMETER; WATER CONTENT; WATER POTENTIAL; WATER RELEASE TIME

Document Type: Research Article

Publication date: September 1, 2006

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more