Cold hardiness of diapause and non-diapause larvae of the parasitoid Colpoclypeus florus Walker (Hymenoptera: Eulophidae) was examined in the laboratory. Mean supercooling point (SCP) for diapausing larvae was −26.7°C and for non-diapausing larvae immediately after their larval development, was −16.2°C. Mean SCP for non-diapause larvae at the prepupa stage was −19.1°C. A short period of acclimation (1 week at 5°C) had no influence on the mean SCPs of both diapause and non-diapause larvae. Pre-freeze mortality for diapause and non-diapause larvae was also studied. A constant exposure of diapause larvae to −6°C resulted in high mortality (70.7%) after a period of 40 days. In contrast, 6 days at −6°C were sufficient to cause the same level of mortality in non-diapause larvae. After exposure of 15 days at −9°C, mortality for non-diapause larvae was 70%, whereas after 20 days at the same temperature mortality of diapause larvae was 25%. The importance of these findings for the cryobiology of C. florus is discussed.
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Document Type: Research Article
November 1, 2005
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.