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Free Content Effect of Cryohypobiosis on Conservation of Human Blood Leukocytes

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Morphological and functional indicators were investigated of donor leukocyte concentrates mixed at a ratio 1:1 with three versions of cold-protective and kept at an undercooling temperature (-10°C) for 24 hours, and with a cryoprotector of optimal composition for 12 days. An optimal version of a non-freezing cold-protective solution was found. It was determined that 76 ± 4 % of the neutrophils retain there ability of phagocytosis among the viable cells (81 ± 5 %) after a 12-day hypobiosis. Thus the results testify to a high morphofunctional safety of human blood leukocytes which experienced cold-hypobiosis (-10°C) in an original non-freezing solution not requiring washing after re-warming.
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Keywords: CRYOHYPOBIOSIS; CRYOPROTECTOR; LEUKOCYTES; NEUTROPHILS

Document Type: Research Article

Publication date: November 1, 2005

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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