Skip to main content
padlock icon - secure page this page is secure

Free Content Cryopreservation of immature seeds of Ponerorchis Graminifolia var. Suzukiana by vitrification

Download Article:
 Download
(PDF 316.3 kb)
 
Ponerorchis graminifolia var. suzukiana is a terrestrial orchid that is an endangered species native to Japan, and it germinates more readily in immature seeds than in mature seeds. To preserve this orchid, an efficient protocol was established for the cryopreservation of immature seeds of P. graminifolia var. suzukiana. When immature seeds of 6 weeks after pollination, which showed higher germination and protocorm formation than mature seeds, were precultured on New Dogashima (ND) medium with 0.3M sucrose for 3 days and cryopreserved by vitrification method (treated with PVS2 for 60 min), the viability after preservation as assessed with 2,3,5-triphenyltetrazolium chloride staining test was about 86%. Immature seeds thus treated showed equal rates of germination and protocorm formation to the untreated control immature seeds, and they developed into normal plantlets on ND medium.

20 References.

No Supplementary Data.
No Article Media
No Metrics

Keywords: CRYOPRESERVATION; ENDANGERED SPECIES; IMMATURE SEEDS; PONERORCHIS GRAMINIFOLIA VAR. SUZUKIANA; VITRIFICATION

Document Type: Regular Paper

Publication date: May 1, 2005

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more