Skip to main content
padlock icon - secure page this page is secure

Free Content Recovery of cryopreserved embryogenic cultures of maritime pine - effect of cryoprotectant and suspension density

Download Article:
 Download
(PDF 270.7 kb)
 
Embryogenic cultures were obtained from seeds of open-pollinated maritime pine trees representing part of a breeding population. The aim of the present study was to develop and optimize a protocol for cryopreservation of Pinus pinaster somatic embryogenic tissue. The density of the embryogenic suspension and the type of pre-treatment significantly affected the recovery of cryopreserved embryogenic cultures, as evaluated by their survival and regrowth rate. An initial density of the suspension culture of 250 mg/ml improved the regrowth rate of the embryogenic lines. Pre-treatment with maltose 0.4 M significantly increased the regrowth rate when compared to the other tested carbohydrates. Also, the addition of Me2SO in a mixture of PEG 4000 and sucrose (PSD solution), instead of Me2SO alone, at the same final concentration, was clearly beneficial for recovery of cryopreserved tissues. This improved method for the cryopreservation of P. pinaster embryogenic of cultures allowed the successful recovery of 97% of the lines stored in liquid of nitrogen.

29 References.

No Supplementary Data.
No Article Media
No Metrics

Keywords: CRYOPRESERVATION; CRYOPROTECTANT; PINUS PINASTER; SOMATIC EMBRYOGENESIS; SUSPENSION DENSITY

Document Type: Regular Paper

Publication date: 01 September 2004

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more