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Free Content Improved Methods for Controlled Rapid Cooling of Cell Suspensions

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Simple, reproducible, methods of achieving rapid rates of cooling in the range 100°C min-1 to 1000°C min-1 are described for straws and cryovial. These methods use the direct contact of straws or cryovials with pre-cooled granules or plates as the heat sink. Liquid nitrogen may be adsorbed into suitable material, for example activated charcoal, zeolites and molecular sieves as the matrix to achieve rapid cooling. Controllable rapid rates of cooling may also be attained by using non-adsorbants. The rate of cooling may be modified by changing the adsorbant material, the size of the adsorbant granules and the temperature of the adsorbant.

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Keywords: CRYOPRESERVATION; CRYOSEM; RAPID COOLING RATES

Document Type: Regular Paper

Publication date: June 1, 2004

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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