Helianthus tuberosus cell suspension cultures were subjected to cryopreservation 24h preculture treatments with 0.5M sucrose or mannitol. Extracts were assayed for transglutaminase activity and the level of a-tubulin tyrosination. There was a significant reduction (compared with the non-precultured controls) in transglutaminase activity and atubulin tyrosination state after mannitol preculture treatment, whereas sucrose preculture treatment produced no significant effect. The results suggest that reduced levels of transglutaminase activity and a-tubulin tyrosination are associated with a lack of post-thaw recovery observed following mannitol preculture treatment of cell culture suspensions. These activities may represent useful molecular markers of the success of preculture treatments in cryopreservation protocols.
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Document Type: Regular Paper
Publication date: 01 May 2004
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.