Skip to main content
padlock icon - secure page this page is secure

Free Content Cryopreservation of Helianthus Tuberosus Cell Suspension Cultures: The Effect of Preculture Treatments on Cytoskeletal Proteins and Transglutaminase Activity

Download Article:
(PDF 31.8 kb)
Helianthus tuberosus cell suspension cultures were subjected to cryopreservation 24h preculture treatments with 0.5M sucrose or mannitol. Extracts were assayed for transglutaminase activity and the level of a-tubulin tyrosination. There was a significant reduction (compared with the non-precultured controls) in transglutaminase activity and atubulin tyrosination state after mannitol preculture treatment, whereas sucrose preculture treatment produced no significant effect. The results suggest that reduced levels of transglutaminase activity and a-tubulin tyrosination are associated with a lack of post-thaw recovery observed following mannitol preculture treatment of cell culture suspensions. These activities may represent useful molecular markers of the success of preculture treatments in cryopreservation protocols.
No References for this article.
No Supplementary Data.
No Article Media
No Metrics


Document Type: Regular Paper

Publication date: May 1, 2004

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more