Freeze-drying of mononuclear cells and whole blood of human cord blood
The recovery of the cells were tested by a haemacytometer, and the highest cell numerical count recovery of MNC was 75.0 ± 4.1% (P<0.01), obtained in the protocol of 40% VP + 20% Sucrose + 10% Mannitol. The viability of the nucleated cells measured by PI staining and the ratio of the number of CD34+ to the number of lymphocytes (by the FITC anti-human CD34+ conjugated antibody method) were measured using a flow cytometer (FCM). The protocol of 40% PVP + 20% Sucrose + 10% fetal bovine serum had the highest viability of 98.57±0.68%(P <0.01). The highest ratio of CD34+ to lymphocytes was 1.17%, and the highest recovery of CD34+ was 68.42 ± 39.5% (P<0.05). Comparing the results of the lyophilized MNC subfraction with that of the whole blood, the lyophilization of the isolated MNC was more successful than that of whole blood.
Document Type: Research Article
Publication date: February 1, 2004
CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.