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Home / Cryoletters, Volume 24, Number 6

Free Content Cracking in a Vitrification Solution During Cooling or Warming Does Not Affect Growth of Cryopreserved Mint Shoot Tips

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Authors: Towill, Leigh E.; Bonnart, Remi

Source: Cryoletters, Volume 24, Number 6, 1 November 2003, pp. 341-346(6)

Publisher: Cryoletters

No obvious decrease in viability or in the ability of mint shoot tips to develop into a shoot occurred during vitrification when the external glass cracked upon either cooling or warming. Samples within semen straws did not show a decrease in survival over three cycles of cooling and warming either in the presence or absence of cracking. No physical defects were visible in treated shoot tips. Cracking of the external glass formed from PVS2 did not obviously influence shoot tip survival in mint species.

Keywords: CRYOPRESERVATION; FRACTURING; MENTHA; REPETITIVE COOLING

Document Type: Research Article

Publication date: 01 November 2003

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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