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Free Content Sucrose preculture to simplify cryopreservation of banana meristem cultures

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A simple cryopreservation method is described for proliferating meristem cultures of banana (Musa spp.). It relies on a 2-week preculture on media containing 0.4 M sucrose followed by rapid cooling in liquid nitrogen. Different preculture media were screened for efficient protection of banana meristems during cryopreservation. Sucrose can be replaced by both fructose and glucose without significantly affecting post-thaw survival. A high BA concentration (100 μM) in the preculture medium results in less material available for cryopreservation, but does not affect cryoprotection. Culture in liquid media significantly improved post-thaw regeneration. The optimized cryopreservation protocol was applied on 36 banana accessions belonging to 8 different genomic groups. It is shown that post-thaw regeneration frequencies (ranging between 0 and 66 %) are highly dependent on the genomic constitution of the banana cultivar.

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Keywords: BANANA; BENZYLADENINE; CRYOPRESERVATION; CRYOPROTECTION; FRUCTOSE; GLUCOSE; MERISTEM CULTURE; MUSA SPP; SUCROSE; SUGAR

Document Type: Research Article

Publication date: November 1, 2002

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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