Skip to main content
padlock icon - secure page this page is secure

Free Content Chondrocyte recovery in cryopreserved porcine articular cartilage after bone carrier alteration

Download Article:
 Download
(PDF 31.7 kb)
 
In order to investigate the consequences on the distribution of cell recovery through a cross-section of articular cartilage, the pathway for ice nucleation and diffusion of water and solutes in porcine osteochondral tissue was altered by drilling a 2mm diameter hole through the subchondral bone to the base of the cartilage. Samples equilibrated with 1M dimethyl sulfoxide were cooled at 1°C/min to -30°C then stored in liquid nitrogen. The most significant change due to bone base modification was an increase in recovery in the middle section of the cartilage, providing insights into mechanisms of cryoinjury in tissue systems.

17 References.

No Supplementary Data.
No Article Media
No Metrics

Keywords: ARTICULAR CARTILAGE; CHONDROCYTE RECOVERY; CRYOPRESERVATION; PORCINE

Document Type: Regular Paper

Publication date: July 1, 2002

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more