Skip to main content
padlock icon - secure page this page is secure

Disruption of ZAS3 in Mice Alters NF-B and AP-1 DNA Binding and T-Cell Development

Buy Article:

$46.00 + tax (Refund Policy)

The large zinc finger proteins, ZAS, regulate the transcription of a variety of genes involved in cell growth, development, and metastasis. They also function in the signal transduction of the TGF- and TNF-α pathways. However, the endogenous protein of a representative member, ZAS3, is rapidly degraded in primary lymphocytes, which limits the determination of its physiological function in vitro. Therefore, we have generated mice with targeted disruption of ZAS3. Oligonucleotide-based microarray analyses revealed subtle but consistent differences in the expression of genes, many of which are associated with receptor or signal transduction activities between ZAS3+/+ and ZAS3−/− thymi. Gel mobility shift assays showed altered DNA binding activities of NF-B and AP-1 proteins in ZAS3-deficient tissues, including the thymus. Lymphocyte analysis suggested a subtle but broad function of ZAS3 in regulating T-cell development and activation. In CD3+ ZAS3−/− thymocytes, the CD4/CD8 ratio was decreased and CD69 expression was decreased. In peripheral CD4+ ZAS3−/− lymphocytes we observed an increased number of memory T cells.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: Gene regulation; Signal transduction; T-cell development; Transcription; Zinc finger proteins

Document Type: Research Article

Affiliations: 1: Department of Pediatrics and Center for Cell and Developmental Biology, Columbus Children's Research Institute, Columbus, OH 43205, USA, Molecular, Cellular and Developmental Biology Program, The Ohio State University, Columbus, OH 43210, USA 2: Department of Pathology, The Ohio State University, Columbus, OH 43210, USA 3: Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USA 4: Department of Pediatrics and Center for Cell and Developmental Biology, Columbus Children's Research Institute, Columbus, OH 43205, USA

Publication date: February 1, 2007

More about this publication?
  • Gene Expression The Journal of Liver Research will publish articles in all aspects of hepatology. Hepatology, as a research discipline, has seen unprecedented growth especially in the cellular and molecular mechanisms of hepatic health and disease, which continues to have a major impact on understanding liver development, stem cells, carcinogenesis, tissue engineering, injury, repair, regeneration, immunology, metabolism, fibrosis, and transplantation. Continued research and improved understanding in these areas will have a meaningful impact on liver disease prevention, diagnosis, and treatment. The existing journal Gene Expression has expanded its focus to become Gene Expression The Journal of Liver Research to meet this growing demand. In its revised and expanded scope, the journal will publish high-impact original articles, reviews, short but complete articles, and special articles (editorials, commentaries, opinions) on all aspects of hepatology, making it a unique and invaluable resource for readers interested in this field. The expanded team, led by an Editor-in-Chief who is uniquely qualified and a renowned expert, along with a dynamic and functional editorial board, is determined to make this a premier journal in the field of hepatology.

    From Volume 16, Gene Expression The Journal of Liver Research is Open Access under the terms of the Creative Commons CC BY-NC-ND license.

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more