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Repositioning of an alternative exon sequence of mouse IgM pre-mRNA activates splicing of the preceding intron

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Using a transient expression system of mouse IgM mini-gene constructs in mouse B-cell lines and in fibroblast L cell, we investigated splicing of the IgM transcript. We observed that the efficiency of splicing between exons C4 and Ml (C4-to-Ml splicing), the splicing reaction leading to the production of membrane-bound form (μm) mRNA, was drastically affected by mutations in a specific portion of the downstream exon (M2). The results show that the specific exon M2 sequence activates the C4-to-Ml splicing. This activation was not observed when splicing between exons Ml and M2 was abolished by base substitutions at the splice sites. These results indicate that positioning of the downstream exon is crucial for efficient splicing of the preceding intron.
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Document Type: Research Article

Publication date: January 1, 1991

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