Characterization of nine polymorphic microsatellite loci in the dyeing poison frog Dendrobates tinctorius (Dendrobatidae), and their cross-species utility in two other dendrobatoid species
While field and laboratory based studies have provided significant insights into the parental care and courtship behaviour of dendrobatoid frogs, a comprehensive assessment of their genetic mating systems and population genetic parameters has been precluded because of the lack of highly variable DNA markers. Here we document the development of nine novel polymorphic microsatellite markers for the dyeing poison frog Dendrobates tinctorius (Dendrobatidae). We found between three and 16 alleles per locus in 60 individuals (30 males, 30 females) from the field site Saut Pararé, French Guiana, with an average observed heterozygosity of 0.75. None of the loci deviated significantly from Hardy–Weinberg equilibrium or showed linkage disequilibrium. We also report successful cross-species amplification of the nine markers in two other dendrobatoid species (Allobates femoralis and Oophaga pumilio). These markers have the potential to aid in determining the genetic structure of local populations, identifying small-scale phylogenies such as parent-offspring relationships and will allow for cross-species comparisons within dendrobatoid species. Therefore, these markers can be applied to a wide range of scientific fields, such as conservation, behavioural ecology and evolutionary biology.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
Document Type: Short Communication
Publication date: October 1, 2012
More about this publication?
- The Herpetological Journal is an international scientific journal that publishes papers on the natural history of amphibians and reptiles. Experimental, observational and theoretical studies are published along with reviews and book reviews. Faunistic lists, letters and results of general surveys are not published unless they shed light on herpetological problems of wider significance.
- Editorial Board
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- Ingenta Connect is not responsible for the content or availability of external websites