Skip to main content
padlock icon - secure page this page is secure

Interaction Of An Amphipathic Peptide Corresponding To Residues 417-434 Of Escherichia Coli Haemolysin With Phospholipid Bilayers

Buy Article:

$68.00 + tax (Refund Policy)

alpha-Haemolysin is an extracellular protein toxin (107KDa) produced by certain pathogenic strains of Escherichia coli. It can bind to lipid bilayers and produce membrane disruption in cell and model membranes. Protein secondary structure predictions suggest an amphipathic helix conformation for the membrane-interacting domain of HlyA, and no potential transmembrane segments. In this paper, we investigated the lipid associating properties of the chemically synthesized peptide H9 (MFEHVASKMADVIAEWEK) corresponding to residues 417-434 of HlyA. Changes in the intrinsic fluorescence and fluorescence anisotropy of the single Trp residue after the addition of DMPC-LUV reveal that the peptide-membrane interaction is optimum at or above the gel-liquid crystalline transition temperature of the lipid. Moreover, the peptide induces vesicle aggregation, as detected through changes in light scattering by the vesicle suspension. However, under comparable conditions, H9 has no hemolytic activity against sheep erythrocytes, nor does it induce leakage of vesicular aqueous contents. These data suggest that HlyA binds the membrane surface through amphipathic helices, though cooperativity among neighbouring helices is probably necessary to explain the lytic effect of the toxin.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: AMPHIPATHIC PEPTIDE; ESCHERICHIA coli HAEMOLYSIN; Haemolytic assays; Intrinsic fluorescence measurements; Merocyanine 540 absorption spectra; Steady state fluorescence anisotropy; a-Haemolysin

Document Type: Review Article

Publication date: December 1, 2001

More about this publication?
  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more