In Vitro Tyrosinase Inhibitory and Antioxidant Activities of Extracts and Constituents of Paeonia lactiflora Pall. Flowers
Method: Ethanol 70% extract of Paeonia lactiflora flowers was partitioned in water and ethyl acetate extracts by liquid/liquid chromatography. These two extracts were purified by combination of chromatographic methods. The chemical structures of the isolated compounds were elucidated on the basis of 1D and 2D NMR spectra and Mass spectrometry. HPLC analysis was used to quantify the major compounds of the ethanol 70% extract. The in vitro fungal tyrosinase assay, using 3,4- dihydroxyphenylalanine (L-DOPA) as substrate, was used to evaluate the antityrosinase potential of these extracts and isolated compounds. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was used to quantifying the antioxidant activity.
Results: Twenty-six compounds were isolated from the flowers of Paeonia lactiflora. The HPLC fingerprint of the ethanol 70% extract of this plant was established. The antioxidant assays revealed that ethanol 70% and ethyl acetate extracts had significant antioxidant abilities in DPPH scavenging activity. The most active compound was identified as methyl gallate. Through the in vitro fungal tyrosinase inhibition screening, we found, that ethanol, ethyl acetate and H2O extracts and three isolated compounds [1,2,3,6-tetra-O-galloyl-β-D-glucopyranoside, 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranoside and kaempferol 3-O-(6-O-galloyl)-β-D-glucopyranoside] inhibit the target protein activities with good IC50 values.
Conclusion: These results demonstrate the antioxidant and antityrosinase activities of ethanol extract of Paeonia lactiflora flowers. A simple, accurate, and reliable method was developed to evaluate the quality of P. lactiflora extracts by using the established HPLC fingerprint and the determination of sixteen compounds. It is interesting that the tetra- and penta-galloylglucoses and the flavonoid have bi-functionality, not just have antioxidative abilities, but also had the inhibitory effects on fungal tyrosinase. These constituents from P. lactiflora exhibited potential applications in medical cosmetology and food supplementation, simultaneously.
Document Type: Research Article
Publication date: September 1, 2017
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