Skip to main content
padlock icon - secure page this page is secure

Multimodal Chromatography for Purification of Biotherapeutics – A Review

Buy Article:

$68.00 + tax (Refund Policy)

Process chromatography forms the core of purification of biotherapeutics. The unparalleled selectivity that it offers over other alternatives combined with the considerable robustness and scalability make it the unit operation of choice in downstream processing. It is typical to have three to five chromatography steps in a purification process for a biotherapeutic. Generally, these steps offer different modes of separation such as ion-exchange, reversed phase, size exclusion, and hydrophobic interaction. In the past decade, multimodal chromatography has emerged as an alternative to the traditional modes. It involves use of more than one mode of separation and typically combines ion-exchange and hydrophobic interactions to achieve selectivity and sensitivity. Over the last decade, numerous authors have demonstrated the significant potential that multimode chromatography offers as a protein purification tool. This review aims to present key recent developments that have occurred on this topic together with a perspective on future applications of multimodal chromatography.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Biopharmaceuticals; Fab; MMC; Multimodal chromatography/mixed-mode chromatography; antibody fragments; host cell proteins; mAb; process related impurities; product related impurities; therapeutic proteins

Document Type: Review Article

Publication date: January 1, 2019

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more