Characterization of Optimized Functional-Complementary Dual Insulinotorpic Peptide rolGG
Methods: A 4rolGG expression plasmid was constructed and expressed in BL21 (DE3). By inducting with IPTG, 4rolGG was expressed at a high level, which was confirmed by SDS-PAGE electrophoresis and Western Blotting. Subsequently, 4rolGG was purified by Ni-NTA affinity chromatography and the purity of 4rolGG was up to 90%.
Result and Conclusion: After oral administration of 4rolGG for 4 weeks, streptozotocin-induced diabetic mice showed a dramatic reduction in the levels of plasma glucose, GHbA1C, TC and TG, while the insulin levels were increased significantly.
Document Type: Research Article
Publication date: August 1, 2017
This article was made available online on December 11, 2017 as a Fast Track article with title: "Characterization of Optimized Functional-Complementary Dual Insulinotorpic Peptide rolGG".
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