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Imaging Conditions Optimization of Human Bronchial Epithelial Cell Based on Atomic Force Microscope

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Background: Surface microstructure of cancerous cell is particularly vital since cell constantly changes its shape as interacting with extracellular matrix and neighboring cells. Atomic force microscope (AFM) has an unparalleled excellence in surface profiling of cells while the scanning quality depends upon experience or experiment conditions primarily.

Methods: In this paper, a quadratic regression orthogonal rotation combination design was conducted to obtain optimal parameters of cell scanning via AFM.

Results: By iterative calculation, the optimum AFM scanning of cell can be achieved at setpoint of 0.61 V, scanning rate of 2.23 Hz and proportional gain of 3.85. Satisfactory surface morphology images of human bronchial epithelium BEAS- 2B were acquired at this calculated scanning condition, in which the details of surface micro cilia structure and the pore structure are visible.

Conclusion: Thus AFM is an effective means for real-time visualization of cell topography in liquid environment and this emerging insight into these cell profiling may promote the understanding of the underlying mechanism for cellular inner reconstruction and cell migration.
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Keywords: Atomic force microscopy; cell profiling; human bronchial epithelium; quadratic regression orthogonal rotation design; scanning condition

Document Type: Research Article

Publication date: February 1, 2016

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  • Current Nanoscience publishes authoritative reviews and original research reports, written by experts in the field on all the most recent advances in nanoscience and nanotechnology. All aspects of the field are represented including nano- structures, synthesis, properties, assembly and devices. Applications of nanoscience in biotechnology, medicine, pharmaceuticals, physics, material science and electronics are also covered. The journal is essential to all involved in nanoscience and its applied areas.
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