Skip to main content
padlock icon - secure page this page is secure

The Interaction between Human Serum Albumin and Cholesterol-modified Pullulan Nanoparticle

Buy Article:

$68.00 + tax (Refund Policy)

The interaction between human serum albumin and cholesterol-modified pullulan (CHP) nanoparticles with different degrees of substitution (DS) of cholesterol moiety was investigated using spectroscopic and thermodynamic methods. Albumin fluorescence intensity was quenched by nanoparticles with maximum emission intensity decreasing at the initial reaction and increasing at the last reacted period. Binding constants (Kb) were 1.12 x 105 M-1, 4.12 x 105 M-1 and 7.44 x 105 M-1 to CHP-3.11, CHP-6.03 and CHP-6.91, respectively, as determined by Stern-Volmer analysis. Adsorption of albumin to nanoparticles was an exothermic reaction process and revealed a higher DS of cholesterol moiety with higher enthalpy and entropy changes. Upon interaction with nanoparticles, albumin conformation changed with a reduction of α-helix, suggesting a partial protein unfolding. Furthermore, albumin could gradually change its helical structure due to the structural change of the complexed nanoparticle. Particle hydrophobicity and shell-core structure play a main role in the alteration of albumin conformation in the nanoparticle-protein interaction process.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Binding constant; fluorescence intensity; helical structure; hydrophobicity; spectroscopic; thermodynamic

Document Type: Research Article

Publication date: December 1, 2012

More about this publication?
  • Current Nanoscience publishes authoritative reviews and original research reports, written by experts in the field on all the most recent advances in nanoscience and nanotechnology. All aspects of the field are represented including nano- structures, synthesis, properties, assembly and devices. Applications of nanoscience in biotechnology, medicine, pharmaceuticals, physics, material science and electronics are also covered. The journal is essential to all involved in nanoscience and its applied areas.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more