Skip to main content
padlock icon - secure page this page is secure

Activation-induced Reorganization in Membrane Nanostructures and Alteration in Adhesion of CD4 + T Lymphocytes Exploited by AFM/LFM

Buy Article:

$68.00 + tax (Refund Policy)

To date, the activation induced reorganization in membrane nanostructures and alteration in membrane adhesion property of CD4 + T lymphocytes largely remain unclear yet even though their immunological functions have been well elucidated. The present work focused on detecting the differences in topography, membrane nanostructures and adhesion/friction behaviors of CD4 + T cells in the absence and presence of stimulus (Phorbol dibutyrate, PDB, plus Ionomycin, ION). The results showed that, due to cell activation in vitro, (a) the formation of pseudopodia, lamellipodia; (b) the appearance of membrane pores with 200∼450 nm in diameter and 70∼110 nm in depth; (c) the formation of nanostructural domains with different adhesion behavior; (d) the loading rate and loading force could affect the measured adhesion force nonlinearly; (e) the dynamic changes in membrane adhesion force, from 348±9.08 pN for resting cells, 827.07±24.61 pN for 24 hours of activation, 372.87±9.26 pN for 48 hours of activation, to 302.45±11.42 pN for 72 hours of activation. This work achieved the biophysical changes of CD4 + T cells with and without stimulation, which would enable us to seek new implications and potential links between cytoarchitectures, membrane adhesion and immunological functions at the single-cell and nanoscale level.

No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: CD4 + T lymphocytes; adhesion behavior; atomic force microscope; cell activation; force curves; force microscopy; in vitro; lateral force; lateral force microscope; membrane nanostructures; topography

Document Type: Research Article

Publication date: June 1, 2011

More about this publication?
  • Current Nanoscience publishes authoritative reviews and original research reports, written by experts in the field on all the most recent advances in nanoscience and nanotechnology. All aspects of the field are represented including nano- structures, synthesis, properties, assembly and devices. Applications of nanoscience in biotechnology, medicine, pharmaceuticals, physics, material science and electronics are also covered. The journal is essential to all involved in nanoscience and its applied areas.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more