Skip to main content
padlock icon - secure page this page is secure

Definition of HILIC System and Quantitative Analysis of Retention Mechanisms

Buy Article:

$68.00 + tax (Refund Policy)

Background: The retention mechanisms of hydrophilic interaction liquid chromatography (HILIC) have been reported as inconclusive and of mixed mode, and have not been explained quantitatively. Packing materials used in HILIC are bare silica gel, polar bonded-silica gels, and ion-exchangers. The chromatography is performed in aqueous solution. Under such condition, the stability of these packing materials is not guaranteed by manufacturers. The fundamental problem associated with such discussions is the lack of definition of experimental conditions, which led to inconclusive discussions.

Objective: To define the experimental conditions, to clarify the differences of HILIC from other liquid chromatography techniques, and to quantitatively explain HILIC retention mechanisms.

Method: Molecular interaction (MI) between an analyte and a simple model phase was quantitatively analyzed in silico in terms of MI energy value difference. MI between an analyte and a solvent molecule was also studied. An analyte was inserted between the model phase and the solvent molecule, and the movement of the analyte was analyzed in terms of the strength of MI energy values.

Results: The analyte moved toward the favorite site based on van der Waals force in reversedphase liquid chromatography, hydrogen bonding in HILIC, and electrostatic interaction in ionexchange liquid chromatography.

Conclusion: For explaining the retention mechanism in HILIC, the experimental condition should be defined, and not be mixed with other liquid chromatographic conditions based on the properties of analytes and packing materials. The HILIC technique described herein is aqueous HILIC, while normal-phase liquid chromatography should be non-aqueous HILIC. Ion-exchange should be clearly classified as an independent liquid chromatographic method.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: HILIC; Hydrophilic interaction liquid chromatography; MOPAC; in silico; modeling; molecular mechanics; retention mechanisms

Document Type: Research Article

Publication date: May 1, 2018

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more