Skip to main content
padlock icon - secure page this page is secure

Quantitative Analysis of Uric Acid Metabolites in Urine by High Performance Liquid Chromatography - Mass Spectrometry Using Silica Hydride Columns

Buy Article:

$68.00 + tax (Refund Policy)

Background: Monitoring of uric acid levels is of significant importance for the prevention of conditions such as gout and kidney disease. Likewise, the metabolites produced from reaction of uric acid with reactive oxygen species are indicative of the level of oxidative stress. Quantitative methods are therefore needed for measuring these compounds in biological sample matrices.

Objective: To investigate the use of silica hydride-based HPLC columns operating in aqueous normal phase mode to retain and separate uric acid from its metabolites in both reference standard solutions and urine samples.

Method: LC-MS instrumentation operating in positive ion mode was used for the detection of the analyte peaks. An internal standard of tyrosine was chosen in order to normalize the detector response. Two stationary phases based on silica hydride were investigated.

Results: The diol-based silica hydride column showed superior chromatographic resolution of the four analyte peaks. As polar molecules, all four were readily retained in the aqueous normal phase mode. Another metabolite, triuret, was also possibly observed, although lack of an available standard precluded definitive confirmation. Quantitative data demonstrated uric acid linearity in the range 0.2–1.6 mg/dL.

Conclusion: The diol functional groups appeared to impart beneficial secondary selectivity to the separation, which was not observed using the other silica hydride-based column. The preliminary quantitative data showed that the developed method approach could be suitable for the analyses of uric acid and its metabolites in biological samples.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: 6-aminouracil; allantoin; aqueous normal phase; diol; hyperuricemia; silica hydride; uric acid

Document Type: Research Article

Publication date: May 1, 2017

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more