Skip to main content
padlock icon - secure page this page is secure

Optimization of fructooligosaccharides synthesis by immobilized fructosyltransferase

Buy Article:

$68.00 + tax (Refund Policy)

The optimization of fructooligosaccharides (FOS) synthesis by immobilized fructosyltransferase from Rhodotorula sp. LEB-V10 was carried out on the basis of the experimental factorial design methodology, consisting of two consecutives (24-1) Fractional Factorial Design (FFD) for the following variable factors: temperature, pH, initial sucrose concentration and immobilized enzyme activity. The main target responses were FOS yield and FOS composition. It was observed that the increase of the immobilized enzyme activity resulted not only in a significant increase of the amount of nystose (GF3) in the FOS mixture but, it also increased the residual fructose concentration in the medium over time. Based on the results from the two FFD, the best conditions for pH and enzyme activity were 6.0 and 20 Ui/mL respectively. The final study step allowed the best conditions for synthesis temperature and sucrose concentration, being 48°C and 50% (w/v), respectively. Under these optimized conditions the total synthesis time was reduced from 96 h to 24 h, the conversion of sucrose was increased by 5% (YFOS = 0.58) and the composition of GF3 by 40% (φGF3 = 0.035).
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: 1F-fructofuranosyl-nystose; Aureobasidium pullulans; Bacillus macerans; Enzymatic synthesis; FOS; Fractional Factorial Design; Ptotaminobacter rubrum; fructosyltransferase; immobilized fructosyltransferase; niobium; optimization

Document Type: Research Article

Publication date: March 1, 2012

More about this publication?
  • Current Chemical Biology aims to publish full-length and mini reviews on exciting new developments at the chemistry-biology interface, covering topics relating to Chemical Synthesis, Science at Chemistry-Biology Interface and Chemical Mechanisms of Biological Systems.

    Current Chemical Biology covers the following areas: Chemical Synthesis (Syntheses of biologically important macromolecules including proteins, polypeptides, oligonucleotides, oligosaccharides etc.; Asymmetric synthesis; Combinatorial synthesis; Diversity-oriented synthesis; Template-directed synthesis; Biomimetic synthesis; Solid phase biomolecular synthesis; Synthesis of small biomolecules: amino acids, peptides, lipids, carbohydrates and nucleosides; and Natural product synthesis).

    Science at Chemistry-Biology Interface (Chemical informatics; Macromolecular catalysts and receptors; Enzymatic synthesis; Biosynthetic engineering; Combinatorial biosynthesis; Plant cell based chemistry; Bacterial and viral cell based chemistry; Chemistry of cellular processes in plants/animals; Receptor chemistry; Cell signaling chemistry; Drug design through understanding of disease processes; Synthetic biology; New high throughput screening techniques; Small molecular array fabrication; Chemical genomics; Chemical and biological approaches to carbohydrates proteins and nucleic acids design; Chemical and biological regulation of biosynthetic pathways; and Unnatural biomolecular analogs).
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more