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The Mechanism of Interacting Biologically Active Complexes Dehydroepiandrosterone- or Tetrahydrocortisol-Apolipoprotein A-I with DNA and their Role in Enhancement of Gene Expression and Protein Biosynthesis in Hepatocytes

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Dehydroepiandrosterone (DHEA), dehydroepiandrosterone-sulfate (DHEAS) and tetrahydrocortisol (THC) with apolipoprotein A-I form the biologically active complexes able to interact specifically with eukaryotic DNA. This conjugate is highly cooperative and results in local splitting of DNA. Specific binding sites of steroid-apoA-I complexes are the (GCC/GGC)n sequences. At the sites of splitting, single-stranded regions sensitized to the action of S1-nuclease form. These regions are irregularly distributed over DNA. The formation of single-stranded DNA regions can promote the interaction with RNA-polymerase. Formation of the biologically active THC (DHEA)-apoA-I complexes is related with resident macrophages having 5α- and 5 β-reductase activity. These complexes greatly enhance the rate of protein biosynthesis in hepatocytes. The cortisol-apoA-I complex does not show such effect. So, the reduced forms of fascicular zone and reticular cortex adrenal zone hormones have synergism of action toward gene expression and protein biosynthesis. The intensification of tissues regeneration during the stress period as a result of given mechanism is discussed.





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Keywords: 3-keto group; BandScan program; Biologically Active Complexes; DNA; DNA structure; Endocrinology; Gene Expression; High density lipoproteins; Protein biosynthesis; RNA-polymerase; Radioactivity; UV-spectrum; X-ray Scattering; X-ray film; adrenal cortex; adrenal cortex fascicular zone; agarose gel. UV-photo; alcohol-ether; apolipoprotein AI; autoradiographs; bacteriophage; blood serum; buffer; chylomicrones; cortisol; dehydroepiandrosterone; densitometry data; electrophoresis; ether-ethanol mixture; eukaryotic; fascicular zones; gentamycin; glucocorticoids; gluconeogenesis enzymes; hepatocyte culture; hepatocytes; hormones; hybridization; hybridization of DNA; hydroxyl group; hypersensitive; leucine solution; luteinizing hormone; macromolecular; macrophages; optical density; reticular; ribosomal RNA; steroid hormones; stoichiometry; sulfate; tetrahydrocortisol; ultracentrifugation

Document Type: Research Article

Publication date: January 1, 2011

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  • Current Chemical Biology aims to publish full-length and mini reviews on exciting new developments at the chemistry-biology interface, covering topics relating to Chemical Synthesis, Science at Chemistry-Biology Interface and Chemical Mechanisms of Biological Systems.

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