Introduction of an Artificial Cu Binding Site at the Surface of CA II: Pitfalls of Rational Design Finally Scooped by Serendipity
Keywords: Artificial Cu; Cambridge Structural Database; Carbonic anhydrase II; Cloning; Design of Mutants; Healthcare; Huisgen reaction; Lysis; Protein Purification; Pseudomonas aeruginosa; Rational Design; Stedium Biotech; Structural Investigation of CA II; X-ray crystallography; Zn2+ ion; active site; amino acids; anaerobic conditions; artificial Cu2+; artificial metal; axial position; binding pocket; binding site; biohybrid materials; carbon dioxide; carbonyl groups; catalytic reaction; catalyzes; copper-ion; crystallographic study; cysteine; cysteine residue; density; disulfide bond; disulfide bridge; enzyme; equilibrium rate; glutathione-S-transferase; helix-helix interactions; high flexibility; high-affinity metal; histidines; hydration; hypothesis; inhibitor cocktail tablet; isothermal titration calorimetry; linear coordination site; melting temperature; metal chelation; metal-center; mutagenesis; novel residues; p-chloromercuribenzoic acid; protein crystallography; protein monomers; protons; site-directed mutagenesis; stoichiometry; target protein; thiole group; triazole; trigonal planar geometry; trigonal-planar coordination; wild-type structures; zinc metalloenzyme
Document Type: Research Article
Publication date: January 1, 2011
- Current Chemical Biology aims to publish full-length and mini reviews on exciting new developments at the chemistry-biology interface, covering topics relating to Chemical Synthesis, Science at Chemistry-Biology Interface and Chemical Mechanisms of Biological Systems.
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