Skip to main content
padlock icon - secure page this page is secure

Introduction of an Artificial Cu Binding Site at the Surface of CA II: Pitfalls of Rational Design Finally Scooped by Serendipity

Buy Article:

$68.00 + tax (Refund Policy)

The aim of this study was the introduction of an artificial copper center at the surface of carbonic anhydrase II (CA II) which subsequently might be used by azide and alkyne building blocks to catalyze triazole formation following 2+3 dipolar cycloaddition. Several mutants were produced to introduce favorable copper coordinating residues taking model copper-proteins from nature as reference templates. Unfortunately, the embarked rational design strategy remained unsuccessful which underlines that our still rather limited understanding of amino acid exchanges on the architecture of proteins might provoke unpredictable effects while tampering with complex biological systems such as a highly functionalized proteins. Finally, more by serendipity than design the formation of an artificial Cu center at the surface of CA II could be achieved. The metal ion is coordinated by two histidines and a serine residue in square planar coordination geometry.





No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Artificial Cu; Cambridge Structural Database; Carbonic anhydrase II; Cloning; Design of Mutants; Healthcare; Huisgen reaction; Lysis; Protein Purification; Pseudomonas aeruginosa; Rational Design; Stedium Biotech; Structural Investigation of CA II; X-ray crystallography; Zn2+ ion; active site; amino acids; anaerobic conditions; artificial Cu2+; artificial metal; axial position; binding pocket; binding site; biohybrid materials; carbon dioxide; carbonyl groups; catalytic reaction; catalyzes; copper-ion; crystallographic study; cysteine; cysteine residue; density; disulfide bond; disulfide bridge; enzyme; equilibrium rate; glutathione-S-transferase; helix-helix interactions; high flexibility; high-affinity metal; histidines; hydration; hypothesis; inhibitor cocktail tablet; isothermal titration calorimetry; linear coordination site; melting temperature; metal chelation; metal-center; mutagenesis; novel residues; p-chloromercuribenzoic acid; protein crystallography; protein monomers; protons; site-directed mutagenesis; stoichiometry; target protein; thiole group; triazole; trigonal planar geometry; trigonal-planar coordination; wild-type structures; zinc metalloenzyme

Document Type: Research Article

Publication date: January 1, 2011

More about this publication?
  • Current Chemical Biology aims to publish full-length and mini reviews on exciting new developments at the chemistry-biology interface, covering topics relating to Chemical Synthesis, Science at Chemistry-Biology Interface and Chemical Mechanisms of Biological Systems.

    Current Chemical Biology covers the following areas: Chemical Synthesis (Syntheses of biologically important macromolecules including proteins, polypeptides, oligonucleotides, oligosaccharides etc.; Asymmetric synthesis; Combinatorial synthesis; Diversity-oriented synthesis; Template-directed synthesis; Biomimetic synthesis; Solid phase biomolecular synthesis; Synthesis of small biomolecules: amino acids, peptides, lipids, carbohydrates and nucleosides; and Natural product synthesis).

    Science at Chemistry-Biology Interface (Chemical informatics; Macromolecular catalysts and receptors; Enzymatic synthesis; Biosynthetic engineering; Combinatorial biosynthesis; Plant cell based chemistry; Bacterial and viral cell based chemistry; Chemistry of cellular processes in plants/animals; Receptor chemistry; Cell signaling chemistry; Drug design through understanding of disease processes; Synthetic biology; New high throughput screening techniques; Small molecular array fabrication; Chemical genomics; Chemical and biological approaches to carbohydrates proteins and nucleic acids design; Chemical and biological regulation of biosynthetic pathways; and Unnatural biomolecular analogs).
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more