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N-Terminal Specific Fluorescence Labeling and its Use in Local Structure Analysis of Proteins (Invited Review)

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The function of a protein correlates closely with its structure; even partial structural disorder/misfolding may lead to various diseases such as Parkinson's disease. Therefore, analysis of the local structure of a protein (e.g., detecting conformation changes during folding/unfolding, and obtaining the information on local polarity, viscosity, etc.) is of great importance for proteomics studies, and in this respect fluorescence spectroscopy plays a crucial role because of its great temporal and spatial sampling capability. In particular, fluorescent probes combined with a site-specific labeling technique have been widely used in structural analysis of proteins due to their powerful ability in the elucidation of various properties and functions of proteins. The developments of excellent spectroscopic probes as well as site-specific labeling methods are the prerequisites for conducting such a kind of study. Herein we review the progress and use of N-terminal specific labeling and fluorescence probes in local structure analysis of proteins, including some results of our recent studies on α-lactalbumin, β-lactoglobulin, and a dimeric protein of DsbC.

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Keywords: N-Terminal specific labeling; fluorescent probes; local structure analysis; proteins

Document Type: Research Article

Publication date: September 1, 2008

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  • Current Chemical Biology aims to publish full-length and mini reviews on exciting new developments at the chemistry-biology interface, covering topics relating to Chemical Synthesis, Science at Chemistry-Biology Interface and Chemical Mechanisms of Biological Systems.

    Current Chemical Biology covers the following areas: Chemical Synthesis (Syntheses of biologically important macromolecules including proteins, polypeptides, oligonucleotides, oligosaccharides etc.; Asymmetric synthesis; Combinatorial synthesis; Diversity-oriented synthesis; Template-directed synthesis; Biomimetic synthesis; Solid phase biomolecular synthesis; Synthesis of small biomolecules: amino acids, peptides, lipids, carbohydrates and nucleosides; and Natural product synthesis).

    Science at Chemistry-Biology Interface (Chemical informatics; Macromolecular catalysts and receptors; Enzymatic synthesis; Biosynthetic engineering; Combinatorial biosynthesis; Plant cell based chemistry; Bacterial and viral cell based chemistry; Chemistry of cellular processes in plants/animals; Receptor chemistry; Cell signaling chemistry; Drug design through understanding of disease processes; Synthetic biology; New high throughput screening techniques; Small molecular array fabrication; Chemical genomics; Chemical and biological approaches to carbohydrates proteins and nucleic acids design; Chemical and biological regulation of biosynthetic pathways; and Unnatural biomolecular analogs).
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