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Isolation and Identification of Certain Phenolic Compounds from Salix mucronata Leaf Extracts and Evaluation Them as Antimicrobial Agents

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Background: In recent years, plant extracts are considered as an important source of many drug formulations for treatment of human beings from infection diseases. The objective of this study was to evaluate the antimicrobial activity of Salix mucronata leaves extracts and isolate their bioactive phytochemicals.

Methods: The dry powder of Salix mucronata was extracted with different aqueous methanol concentrations. The 85% methanolic extract was further fractionated using different organic solvents. The antimicrobial activity of different extracts and fractions was evaluated. The most bioactive fractions were submitted for chromatographic isolation and structure elucidation of their phytochemicals using chromatographic and spectroscopic methods.

Results: The ethyl acetate and the butanolic fractions derived from 85% MeOH extract gave a high antimicrobial activity with inhibition zones ranging between 10 mm and 26 mm and minimum inhibitory concentration (MIC) value of 8 mg/mL. While the butanolic fraction showed zones of inhibition ranging between 10 mm and 25 mm with MIC 8 mg/mL. Six compounds were isolated from ethyl acetate fraction and their structures were elucidated as; apigenin (1), quercetin (2), quercetrin (3), rhamnazin -3-O-β-D-glucopyranoside (4), Chrysoeriol-7-O-β-D-glucuronoid- 6 -methyl ester (5), and tremuloidin (6). Also, five compounds were isolated from the butanolic fraction and their structures were elucidated as; kaempferol (7), luteolin (8), luteolin-3 - methoxy-4 - O-β-D- glucopyranoside (9), isorhamnetin -3-O-β -D-glucopyranoside (10) and salicin (11).

Conclusion: The results of the present study showed that the ethyl acetate and the butanolic fractions contain high flavonoids and salicinoids compounds which may attribute to their potential as antimicrobial agents.
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Keywords: Antimicrobial properties; Salix mucronata; chromatography; flavonoids; phenolic compounds; salicinoids

Document Type: Research Article

Publication date: June 1, 2019

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  • The journal aims to provide updates to researchers about new bioactive compounds with proven activities in various biological screenings and pharmacological models. The journal will contain information about the structures, biological activities and sources of chemical entities discovered or designed by researchers and published in leading journals. The aim is to provide a valuable information source of bioactive compounds synthesized or isolated, which can be used for further development of pharmaceuticals by industry and academia.

    The journal should prove to be essential reading for pharmacologists, pharmaceutical chemists and medicinal chemists who wish to be kept informed and up-to-date with the latest and most important developments about new bioactive compounds of natural or synthetic origin, including recent patents.
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