Skip to main content
padlock icon - secure page this page is secure

In Vitro Antioxidant and Acetylcholinesterase Inhibitory Activities of Fractions from Centella asiatica (Linn.) Extract

Buy Article:

$68.00 + tax (Refund Policy)

Background: Alzheimer disease is a neurological disease related to age which is characteristic of excess of oxidative stress and deficit level of acetylcholine in the brain. Centella asiatica (Linn.) have several beneficial pharmacological activities such as antioxidant, anti-inflammatory and memory enhancing activities.

Objective: To evaluate the phenolic contents, antioxidant and AChE inhibitory activities of different fractions of Centella asiatica grown in Vietnam.

Methods: The leaves of Centella asiatica were extracted with ethanol 96% and fractionated with nhexane, ethyl acetate and butanol solvents. These fractions were investigated the antioxidant activity by DPPH assay and acetylcholinesterase (AChE) inhibitory activity by Ellman's colorimetric method.

Results: Our results showed that the total phenolic content of BuOH fraction was the highest, being equivalent to 68.4 ± 2.4 mg quercetin/g of fraction. Our data also demonstrated that BuOH fraction had the strongest antioxidant activity with IC50 of 7.76 ± 0.74 μg/mL and AChE inhibitory activity with an IC50 value of 36.31 ± 2.45 μg/mL in a concentration-dependent manner, followed by EtOAc fraction and nhexane fraction with the weakest activity. Kinetic inhibition analysis indicated BuOH fraction of mixed inhibition type with Ki (representing the affinity of the enzyme and inhibitor) of 34.36 ± 2.34 μg/mL.

Conclusion: Our data suggest that the BuOH fraction of Centella asiatica may be a promising source of AChE inhibitors for Alzheimer's disease.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Centella asiatica; acetylcholinesterase; antioxidant; extraction; inhibitory; oxidative stress

Document Type: Research Article

Publication date: March 1, 2018

More about this publication?
  • The journal aims to provide updates to researchers about new bioactive compounds with proven activities in various biological screenings and pharmacological models. The journal will contain information about the structures, biological activities and sources of chemical entities discovered or designed by researchers and published in leading journals. The aim is to provide a valuable information source of bioactive compounds synthesized or isolated, which can be used for further development of pharmaceuticals by industry and academia.

    The journal should prove to be essential reading for pharmacologists, pharmaceutical chemists and medicinal chemists who wish to be kept informed and up-to-date with the latest and most important developments about new bioactive compounds of natural or synthetic origin, including recent patents.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more