Anti-leishmanial Activity of Gossypium hirsutum L., Ferula assa-foetida L. and Artemisia aucheri Boiss. Extracts by Colorimetric Assay
Aim: Plant extracts or plant-derived compounds are likely to provide a valuable source of new medicinal agents. In the present work anti-leishmanial activity of methanolic extracts of Gossypium hirsutum, Ferula assa-foetida and Artemisia aucheri, was studied on Leishmania major promastigotes
by colorimetric assay in comparison to a trivalent antimony compound (tartar emetic). Methods: L. major promastigotes were cultured at 24±1°C to stationary phase in RPMI 1640 medium, supplemented with 10% heat inactivated fetal calf serum (FCS) and antibiotics. The biological activity
of three plant extracts was studied against L. major promastigotes in comparison to tartar emetic. The optical density (OD) due to cleavage of the tetrazolium salt MTT [3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] into a colored product formazan by the parasite was measured
using an ELISA reader, to determine IC50 values (50% inhibitory concentrations). All experiments were repeated in triplicate. Results: All three plant extracts and tartar emetic inhibited the growth of promastigote stage of L.major after 72 hours of incubation. Tartar emetic as positive control
gave a 50% inhibitory concentration (IC50) of 4.7µg/ml, while the IC50 values of G. hirsutum, F. assa-foetida and A. aucheri, were determined as 3.6, 5.9 and 7.5µg/ml, respectively. Although G. hirsutum was more active than the others, but all extracts had profound effects on promastigotes
of L. major. Conclusion: This study provides valuable data on potent activity of three plant extracts. Further works are required to evaluate the leishmanicidal effect of these plant extracts on animal models or human volunteers.
Keywords: Artemisia aucheri; Ferula assa-foetida; Gossypium hirsutum; Leishmania major; MTT assay
Document Type: Research Article
Publication date: 01 July 2014
- Anti-Infective Agents publishes original research, expert reviews, drug clinical trial studies and guest edited issues on all the latest and outstanding developments on the medicinal chemistry, biology, pharmacology and use of anti-infective and anti-parasitic agents. The scope of the journal covers all pre-clinical and clinical research on antimicrobials, antibacterials, antiviral, antifungal, and antiparasitic agents. Anti-Infective Agents is an essential journal for all infectious disease researchers in industry, academia and the health services.
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