Non-Enzymatic Electrochemical Immunosensor for the Detection of Botulinum Neurotoxin Serotype-B
We developed electrochemical immunosensor for the non-enzymatic detection of botulinum neurotoxin-B (BoNT/B) based on gold nanoparticles (Au NPs) loaded on boron nitride nanostructures (BN) i.e., as signal labels. The synthesized BN-Au NPs were characterized by transmission electron microscopy (TEM), energy-dispersive X-ray (EDX), cyclic voltammetry (CV) and ultra violet-visible (Uv-vis) spectroscopy. The immunosensor was prepared by immobilization of rabbit anti-BoNT/B antibodies (Ab1) on cobalt phthalocyanine-chitosan (CoPC-CS) modified glassy carbon electrodes followed by blocking the surface with bovine serum albumin to minimize nonspecific binding. Further, the electrodes were exposed to the sample containing BoNT/B sample (spiked buffer or spiked food sample). In the next step, mouse anti-BoNT/B antibodies (Ab2) conjugated BN-Au NPs was used as non-enzymatic signal label for the redox cyclic signal amplification based approach for quantification of BoNT/B. The prepared immunosensor could detect BoNT/B over a wide concentration range from 20 pg/mL to 100 ng/mL and detection limits of 10 pg/mL obtained for differential pulse voltammetric (DPV) measurements. The developed method was highly selective to detect BoNT/B in the presence of other serotypes of BoNT.
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Document Type: Research Article
Publication date: November 1, 2017
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