Skip to main content
padlock icon - secure page this page is secure

Non-Enzymatic Electrochemical Immunosensor for the Detection of Botulinum Neurotoxin Serotype-B

Buy Article:

$106.46 + tax (Refund Policy)

We developed electrochemical immunosensor for the non-enzymatic detection of botulinum neurotoxin-B (BoNT/B) based on gold nanoparticles (Au NPs) loaded on boron nitride nanostructures (BN) i.e., as signal labels. The synthesized BN-Au NPs were characterized by transmission electron microscopy (TEM), energy-dispersive X-ray (EDX), cyclic voltammetry (CV) and ultra violet-visible (Uv-vis) spectroscopy. The immunosensor was prepared by immobilization of rabbit anti-BoNT/B antibodies (Ab1) on cobalt phthalocyanine-chitosan (CoPC-CS) modified glassy carbon electrodes followed by blocking the surface with bovine serum albumin to minimize nonspecific binding. Further, the electrodes were exposed to the sample containing BoNT/B sample (spiked buffer or spiked food sample). In the next step, mouse anti-BoNT/B antibodies (Ab2) conjugated BN-Au NPs was used as non-enzymatic signal label for the redox cyclic signal amplification based approach for quantification of BoNT/B. The prepared immunosensor could detect BoNT/B over a wide concentration range from 20 pg/mL to 100 ng/mL and detection limits of 10 pg/mL obtained for differential pulse voltammetric (DPV) measurements. The developed method was highly selective to detect BoNT/B in the presence of other serotypes of BoNT.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: BORON NITRIDE NANOSTRUCTURES; BOTULINUM NEUROTOXIN; ELECTROCHEMICAL IMMUNOSENSOR; GOLD NANOPARTICLES; NON-ENZYMATIC LABEL

Document Type: Research Article

Publication date: November 1, 2017

More about this publication?
  • The growing interest and activity in the field of sensor technologies requires a forum for rapid dissemination of important results: Sensor Letters is that forum. Sensor Letters offers scientists, engineers and medical experts timely, peer-reviewed research on sensor science and technology of the highest quality. Sensor Letters publish original rapid communications, full papers and timely state-of-the-art reviews encompassing the fundamental and applied research on sensor science and technology in all fields of science, engineering, and medicine. Highest priority will be given to short communications reporting important new scientific and technological findings.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more