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New Medium for Rapid Quantification of Micrococcus Luteus Cells by Multichannel Series Piezoelectric Quartz Crystal Based Method

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The pathogenicity of Micrococcus luteus has become increasingly prominent in immunocompromised patients. This bacterium can cause illness even in healthy people when it reaches the certain number. But so far few reports have been published about how to quantify the viable cells of Micrococcus luteus. In this paper, a new developed culture medium was proposed for rapid quantification of Micrococcus luteus cells by multichannel series piezoelectric quartz crystal (MSPQC) sensor. Raffinose was used as a carbon source to substitute glucose in the new medium. Good response signal of MSPQC can be obtained even if 0.5% (w/v) of raffinose was used. In the range of 10–107 colony forming units (cfu) per ml, Micrococcus luteus ATCC 4698 can be estimated by proposed method. A total of 59 clinical Micrococcus luteus isolates were also evaluated by proposed method and the accuracy rate was about 93.2%. Comparing with conventional method, the proposed method is simple, rapid and easy to operate.
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Keywords: MEDIUM; MICROCOCCUS LUTEUS; MULTICHANNEL SERIES PIEZOELECTRIC QUARTZ CRYSTAL (MSPQC); QUANTIFICATION; RAFFINOSE

Document Type: Research Article

Publication date: August 1, 2010

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