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Development of Bioselective Element of SPR Spectrometer for Monitoring of Oligonucleotide Interactions and Comparison with Thermodynamic Calculations

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A bioselective element (a specific recognition layer on the sensor surface) of SPR spectrometer for label-free detection of DNA sequence of the hybrid bcr-abl gene is firstly developed using thiol-modified oligonucleotides. Preparation of the bioselective element and subsequent hybridization events were monitored in real time by Surface Plasmon Resonance (SPR) spectrometer "Plasmon SPR-4 m." The thiol-modified single-stranded oligonucleotide mod-Ph is shown to be immobilized efficiently on a gold sensor surface of the SPR spectrometer. Specific hybridization between immobilized mod-Ph and complementary oligonucleotide P1 was detected, whereas no sensor response to non-complementary target was observed. The experimental results on interactions between the oligonucleotides under study are in good agreement with theoretical calculations of thermodynamic parameters of these interactions. The described approach can be proposed as a basis for creating biosensor for real-time label-free diagnostics of some types of leukemia.
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Document Type: Research Article

Publication date: October 1, 2009

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