Skip to main content
padlock icon - secure page this page is secure

Electrical Characterization of Biological Molecules Deposition in MOS Capacitors

Buy Article:

$106.72 + tax (Refund Policy)

The aim of this work was the electrical characterization of biological molecules covalently immobilized on the dielectric of a MOS-like structure. The experimental protocol to bond the biological molecules on SiO2 lies in: oxide activation, silanization, linker molecule deposition, biological molecule bonding. Both an enzyme, the glucose oxidase (GOx), and single-stranded oligonucleotides (ssDNA) were selected as biological molecules. The effectiveness of the immobilization procedure was tested by X-ray Photoelectron spectroscopy. The electrical characterization was carried out on reference and fully processed samples as a function of the electrolyte pH, from 3 to 8, and of measurement time, up to 200 s. The oxide does not experience any aging during the measurement sets for voltages up to ±3 V. GOx deposition produces a shift of about −0.7 ± 0.04 V in the VFB, suggesting the molecule has a positive charge when anchored to the oxide layer. On the other hand, ssDNA deposition causes a positive shift, below 0.3 V, as expected due to the DNA negative charge in solution. The hybridization process causes a further shift in the VFB above 0.4 V, well above the experimental errors, confirming the sensitivity of this device to monitor the hybridization. Our preliminary data show a potential for the development of MOS-based biosensors.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: DNA; ELECTRICAL SENSING; GLUCOSE OXIDASE; IMMOBILIZATION; MOS CAPACITORS; SILICON

Document Type: Research Article

Publication date: August 1, 2008

More about this publication?
  • The growing interest and activity in the field of sensor technologies requires a forum for rapid dissemination of important results: Sensor Letters is that forum. Sensor Letters offers scientists, engineers and medical experts timely, peer-reviewed research on sensor science and technology of the highest quality. Sensor Letters publish original rapid communications, full papers and timely state-of-the-art reviews encompassing the fundamental and applied research on sensor science and technology in all fields of science, engineering, and medicine. Highest priority will be given to short communications reporting important new scientific and technological findings.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more