Skip to main content
padlock icon - secure page this page is secure

A Bio-Sensor for Human Neutrophil Elastase Employs Peptide-p-Nitroanilide Cellulose Conjugates

Buy Article:

$106.72 + tax (Refund Policy)

High levels of human neutrophil elastase (HNE) in chronic wounds have been associated with degradation of cytokine growth factors necessary for normal wound healing. Thus, accurate clinical detection and quantification of HNE will be important to the therapeutic management of chronic wounds. Colorimetric detection of HNE using immobilized HNE substrate-cellulose analogs has been assessed. The chromogenic peptide substrate Succinyl-Ala-Ala-Pro-Ala-pNA and its analog Succinyl-Ala-Ala-Pro-Val-pNA were attached to derivatized cellulose. Cellulose was treated with 3-aminopropyltriethoxysilane to form the amino-propyloxy ether of cellulose. The aminopropyloxy-ether of cellulose (Cell-AP) was reacted with the HNE chromogenic para-nitroanilide peptide substrates to form a covalently linked conjugate of cellulose (Cell-AP-suc-Ala-Ala-Pro-Ala-pNA or Cell-AP-suc-Ala-Ala-Pro-Val-pNA) through amide bond between the Cell-AP amine and the succinyl carboxylate of the substrate. The colorimetric response of the cellulose-bound chromophore was assessed in HNE buffered solutions by monitoring release of para-nitroaniline from the derivatized cellulose probe to determine HNE levels from 5.0 × 10−3 to 6.0 units per ml. A comparison of the analogs rate of hydrolysis demonstrated that Cell AP-suc-Ala-Ala-Pro-Ala-pNA was faster and it gave slightly stronger absorption than the Cell-AP-suc-Ala-Ala-Pro-Val-pNA. Visual differentiation and detection of elastase activity units resulting from substrate hydrolysis was optimal at 2–4 minutes with Cell-AP-suc-Ala-Ala-Pro-Ala-pNA, and at 15–60 minutes with Cell-AP-suc-Ala-Ala-Pro-Val-pNA.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics


Document Type: Research Article

Publication date: August 1, 2008

More about this publication?
  • The growing interest and activity in the field of sensor technologies requires a forum for rapid dissemination of important results: Sensor Letters is that forum. Sensor Letters offers scientists, engineers and medical experts timely, peer-reviewed research on sensor science and technology of the highest quality. Sensor Letters publish original rapid communications, full papers and timely state-of-the-art reviews encompassing the fundamental and applied research on sensor science and technology in all fields of science, engineering, and medicine. Highest priority will be given to short communications reporting important new scientific and technological findings.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more