Skip to main content
padlock icon - secure page this page is secure

Altering Kinetics of Polymerization Can Modulate Mesenchymal Stem Cells Interaction with 3D Matrix

Buy Article:

$106.87 + tax (Refund Policy)

Aim of regenerative medicine and tissue engineering is to provide an alternative approach to tissue harvesting and in order to achieve this biomaterials play pivotal role as these matrices provide a three-dimensional base to the cells, microenvironment for its differentiation and architecture suitable for extra-cellular matrix synthesis leading to neo-tissue generation. However, depending upon the technique for scaffold fabrication different properties could be imparted on these scaffolds and by altering the chemical reaction could have effect on the bio-functionality of the scaffold. With this aim, we report for the first time effect of using free-radical polymerization agents on PLGA-gelatin scaffold synthesized via freeze-thawing technique. Two sets of PLGA-gelatin matrix was fabricated one without redox agent ammonium persulphate (APS) and N, N', N' -tetramethylethylenediamine (TEMED) (PG) and another scaffold using both these agents (PGAT). Scanning electron microscopy image shows significant difference in pore size and pore architecture in both scaffold with PG showing large closed pores with average pore size of 150 μm and PGAT matrix shows smaller interconnected and open pores with average pore diameter of 85 μm. These results were further confirmed by Micro-CT which shows significant difference in the architecture of both the scaffold. Human mesenchymal stem cell cultured on these matrices showed high cellular attachment in both scaffold however, in PG cells were found to be more spindle shaped with high extracellular matrix secretion. Furthermore, hMSCs cultured on PGAT were found to be more circular which indicates more cell–cell interaction than cell-matrix interactions. Goal of this work was to demonstrate that by modulating a simple step during scaffold fabrication can have substantial effect on how cells interacts with these biomimetic.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: CELL-MATRIX CHEMISTRY; GELATIN; HUMAN MESENCHYMAL STEM CELLS; MICRO-CT; PLGA; REDOX AGENT; SCAFFOLD

Document Type: Research Article

Publication date: August 1, 2016

More about this publication?
  • Science of Advanced Materials (SAM) is an interdisciplinary peer-reviewed journal consolidating research activities in all aspects of advanced materials in the fields of science, engineering and medicine into a single and unique reference source. SAM provides the means for materials scientists, chemists, physicists, biologists, engineers, ceramicists, metallurgists, theoreticians and technocrats to publish original research articles as reviews with author's photo and short biography, full research articles and communications of important new scientific and technological findings, encompassing the fundamental and applied research in all latest aspects of advanced materials.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more