Altering Kinetics of Polymerization Can Modulate Mesenchymal Stem Cells Interaction with 3D Matrix
Aim of regenerative medicine and tissue engineering is to provide an alternative approach to tissue harvesting and in order to achieve this biomaterials play pivotal role as these matrices provide a three-dimensional base to the cells, microenvironment for its differentiation and architecture suitable for extra-cellular matrix synthesis leading to neo-tissue generation. However, depending upon the technique for scaffold fabrication different properties could be imparted on these scaffolds and by altering the chemical reaction could have effect on the bio-functionality of the scaffold. With this aim, we report for the first time effect of using free-radical polymerization agents on PLGA-gelatin scaffold synthesized via freeze-thawing technique. Two sets of PLGA-gelatin matrix was fabricated one without redox agent ammonium persulphate (APS) and N, N', N' -tetramethylethylenediamine (TEMED) (PG) and another scaffold using both these agents (PGAT). Scanning electron microscopy image shows significant difference in pore size and pore architecture in both scaffold with PG showing large closed pores with average pore size of 150 μm and PGAT matrix shows smaller interconnected and open pores with average pore diameter of 85 μm. These results were further confirmed by Micro-CT which shows significant difference in the architecture of both the scaffold. Human mesenchymal stem cell cultured on these matrices showed high cellular attachment in both scaffold however, in PG cells were found to be more spindle shaped with high extracellular matrix secretion. Furthermore, hMSCs cultured on PGAT were found to be more circular which indicates more cell–cell interaction than cell-matrix interactions. Goal of this work was to demonstrate that by modulating a simple step during scaffold fabrication can have substantial effect on how cells interacts with these biomimetic.
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Document Type: Research Article
Publication date: August 1, 2016
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