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Photochemical Synthesis of Silver Nanoparticles/Eggshell Membrane Composite, Its Characterization and Antibacterial Activity

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Eggshell membrane (ESM) is a natural biomembrane with two different surfaces of the limiting membrane and the outer shell membrane. In this study, a simple and economical method was developed to synthesize silver nanoparticles (AgNPs) using chicken ESM under simulated sunlight radiation to obtain a nanobiocomposite of AgNPs/ESM. The AgNPs synthesized on both surfaces of the ESM were characterized by visual observation, ultraviolet-visible diffuse reflectance spectroscopy, field-emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, high-resolution transmission electron microscopy and selected-area electron diffraction. The results indicated that the surface structure of ESM affected the synthesis of AgNPs. The synthesized AgNPs were mainly spherical with an average particle size of 10.4 ± 5.6 nm and 23.6 ± 7.8 nm on the limiting membrane and outer shell membrane, respectively. Furthermore, the antibacterial activity of the AgNPs/ESM was evaluated against the Gram-negative bacterium Escherichia coli and the Gram-positive bacterium Staphylococcus aureus using the Kirby–Bauer disk diffusion method. The results showed that the nano-biocomposite possessed good antibacterial properties.
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Document Type: Research Article

Publication date: August 1, 2016

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  • Science of Advanced Materials (SAM) is an interdisciplinary peer-reviewed journal consolidating research activities in all aspects of advanced materials in the fields of science, engineering and medicine into a single and unique reference source. SAM provides the means for materials scientists, chemists, physicists, biologists, engineers, ceramicists, metallurgists, theoreticians and technocrats to publish original research articles as reviews with author's photo and short biography, full research articles and communications of important new scientific and technological findings, encompassing the fundamental and applied research in all latest aspects of advanced materials.
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