Effect of Titanium Dioxide Nanoparticles on Silkworm's Innate Immunity and Resistance to Bacillus bombyseptieus
Titanium dioxide nanoparticles (TiO2 NPs) has been widely used in medicine and biology. Low dose TiO2 NPs can improve silkworm's resistance against virus disease, however, the effect of TiO2 NPs on silkworm innate immunity and bacterial disease has not been reported. In this study, we performed digital gene expression profile (DGE) analysis for silkworm midgut, which revealed that pretreatment with TiO2 NPs caused significant changes in 15 pathways (P<0.05), including tyrosine metabolism and melanogenesis. Results from ontology annotation and pathway analysis indicated that the differentially expressed genes mainly participated in immune response, defense response, energy metabolism, and apoptosis. Silkworms pretreated with TiO2 NPs increased survival rate of 13.30% when infection with Bacillus bombyseptieus (Bb), reduced not only the phenol oxidase cascade but also the activation levels of Toll and Imd induced by Bb. Moreover, TiO2 NPs also inhibited the silkworm apoptosis induced by Bb. The present study is the first one that clarifies the mechanism of TiO2 NPs' effect on silkworm's resistance against Bb, which is through the early activation of the innate immunity. The results presented here provide a new method for the application of TiO2 NPs in silkworm disease prevention.
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Document Type: Research Article
Publication date: August 1, 2016
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